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4x SDS PAGE Reducing Sample Buffer

25mL Milli-Q Water
25mL 1M Tris, pH 6.8
40mL Glycerol
8g SDS
8mL ß-Mercapto-ethanol
400mg Bromophenol Blue

Once SDS and BB are dissolved, filter with a 0.2µm syringe filter.
Store aliquots in -20ºC